The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease through Regulating the Visceral Adipose-Tissue Function

نویسندگان

  • Jeongjun Kim
  • Haerim Lee
  • Jonghoon Lim
  • Jaeho Oh
  • Soon Shik Shin
  • Michung Yoon
چکیده

Similar to neoplastic tissues, growth and development of adipose tissue are thought to be angiogenesis-dependent. Since visceral adipose tissue (VAT) is associated with development and progression of nonalcoholic fatty liver disease (NAFLD), we hypothesized that angiogenesis inhibition would attenuate obesity-induced NAFLD. We fed C57BL/6J mice a low-fat diet (LFD, chow 10% kcal fat), a high-fat diet (HFD, 45% kcal fat) or HFD supplemented with the lemon-balm extract ALS-L1023 (HFD-ALS) for 15 weeks. ALS-L1023 reduced endothelial cell-tube formation in vitro. HFD increased VAT angiogenesis and induced weight gains including body weight, VAT mass and visceral adipocyte size compared with LFD. However, HFD-ALS led to weight reductions without affecting calorie intake compared with HFD. HFD-ALS also reduced serum ALT and AST levels and improved lipid metabolism. HFD-ALS suppressed steatosis, infiltration of inflammatory cells, and accumulation of collagen in livers. HFD-ALS modulated hepatic expression of genes involved in lipid metabolism, inflammation, fibrosis, antioxidation, and apoptosis. Concomitantly, analysis of VAT function revealed that HFD-ALS led to fewer CD68-positive macrophage numbers and lower expression of inflammatory cytokines compared with HFD. Our findings show that the anti-angiogenic herbal extract ALS-L1023 attenuates NAFLD by targeting VAT during obesity, suggesting that angiogenesis inhibitors could aid in the treatment and prevention of obesity-induced human NAFLD.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The effect of aerobic exercise on epicardial adipose tissue, insulin resistance, and some liver enzymes in high-fat diet-induced obesity male wistar rat

Background and Aim: Due to the prevalence and socio-economic consequences of obesity in mortality, cardiovascular (CAD) and nonalcoholic fatty liver disease the effectiveness of aerobic exercise on epicardial adipose tissue (EAT), insulin resistance (IR) and some liver enzymes of high-fat diet-induced obesity male wistar rats was investigated. Methods: Thirty-two male Wistar rats with an averag...

متن کامل

Inhibition of 5-Lipoxygenase inhibitor zileuton in high-fat diet-induced nonalcoholic fatty liver disease progression model

Objective(s): Arachidonic Acid/5-lipoxygenase (AA/5-LOX) pathway connects lipid metabolism and proinflammatory cytokine, which are both related to the development and progression of nonalcoholic fatty liver disease (NAFLD). Therefore, the present study was designed to investigate the role of AA/5-LOX pathway in progression of NAFLD, and the effect of zileuton, an inhibitor of 5-LOX, in this mod...

متن کامل

THE EFFECT OF 12 WEEKS OF MODERATE INTENSITY CONTINUOUS TRAINING (MICT) ON INFLAMMATORY AND ANGIOGENESIS FACTORS OF VISCERAL AND SUBCUTANEOUS ADIPOSE TISSUE IN OBESE RATS: A SEMI-EXPERIMENTAL STUDY

Background & Aims: Physical activity by modulating angiogenesis in adipose tissue, effects on the entire body metabolic profile. Therefore, the aim of this study was to investigate the effect of 12 weeks of moderate intensity continuous training on VEGF, PEDF and PAI-1 of visceral and subcutaneous adipose tissue in obese rats. Materials & Methods: 15 male Wistar rats were divided into 2 groups...

متن کامل

The Effect of Aerobic Continuous Training on Myonectin, Insulin Resistance and Liver Enzymes in Rats with Nonalcoholic Fatty Liver Disease

Background. Non-alcoholic fatty liver disease, is the most common reason of liver disorders that can be controlled by dieting and regular physical activity.  Objectives. The aim of this study was to investigate the effect of aerobic continuous training on serum levels of myonectin, insulin resistance and liver enzymes in rats with non-alcoholic fatty liver disease. Methods. Thirty-four male W...

متن کامل

Momordica cymbalaria fruit extract attenuates high-fat diet-induced obesity and diabetes in C57BL/6 mice

Objective(s): The present study was aimed to evaluate the effect of methanolic fruit extract of Momordica cymbalaria (MeMC) against high-fat diet-induced obesity and diabetes in C57BL/7 mice.Materials and Methods: In the present study, six weeks old male C57BL/6 mice were divided into four groups. G-1 and G-2 served as lean control and HFD control, G-3 and G-4 received MeMC 25 and 50 mg/kg, BW ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 18  شماره 

صفحات  -

تاریخ انتشار 2017